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In the BBB model with iNHA, the bottom of the inserts was precoated with 0.001% Poly-L-Lysine (L 7240, Biochrom AG, Darmstadt, Germany) for 45 min at room temperature

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Anchoring on the per-unit figure early is useful because it turns every subsequent question into a one-step multiplication or division rather than a fresh calculation from raw mass

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