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pH Determination in Water and Culture Medium GHK, GHK-Cu, Cu(OAc) 2 and CuCl 2 samples were prepared and diluted in sterile water and culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin) respectively to a final concentration of 580 M and 5800 M

However, the effect of oxidative and nitrosative stress is not restricted to the intracellular environment, as reactive species can cross cellular membranes and influence extracellular proteins

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Tannins have been shown to reduce oxidative damage to lipids, proteins, and DNA, contributing to their protective effects against various diseases [217]

Hydroxytyrosol inhibits the proliferation of human colon adenocarcinoma cells through Inhibition of ERK1/2 and Cyclin D1