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Firstly, the 0.1 g frozen plant tissue was ground and then placed in a 10 ml tube containing 1 mL phenol extraction buffer and mix, and the sample was held at 25 C for 10 min

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As the community of Kahemba is very familiar with the ongoing research efforts to prevent konzo, these studies may even help de-stigmatize individuals with konzo through determining the biology behind this disease all while attempting to draw global attention to this neglected disease

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These results in agreement with the previous studies