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doi:10.2165/00128072-200204010-00005

For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent

2), indicating that the general mechanism with a separate oxidative and reductive half-reaction was not altered by the mutations

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