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ChemBioChem 27(1):e202500312

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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles

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There are 100 units in 1 milliliter (mL) in a standard U-100 insulin syringe

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Suitable for studies involving melanocortin receptor pathway characterization (e.g., MC1R/MC4R models) and method development in model systems

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