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Eluent A consisted of acetonitrile:water (50:50, v/v, both ULC/MS-CC/SFC grade, Biosolve-Chemicals) and eluent B comprised 2-propanol:acetonitrile:water (85:10:5, v/v/v), both containing 5 mM ammonium formate (MS grade, Sigma-Aldrich) and 0.1% formic acid (ULC/MS-CC/SFC grade, Biosolve-Chemicals)
Vitamin B12 is absorbed from food in the terminal ileum (the last part of the small intestine)
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