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Materials and methods Cell culture The cultivation of HCT116 cells was performed via McCoys 5A medium (Pricella, Wuhan, China), and SW480 cells were maintained in high-glucose DMEM medium (Solarbio, Beijing, China), supplemented with 10% serum (HyClone, USA) and 1% penicillin/streptomycin (Solarbio, Beijing, China)
A recent study showed a strong negative correlation between breast tumor grade progression and GPx4 expression in breast cancer tissues, and its downregulation may be related to the poor prognosis of patients with invasive ductal carcinoma of the breast [106]
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