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Identification of EndMT After cells were challenged with TGF-1-IL-1 for 72 h, cells were observed under a phase contrast microscope (Nikon, Tokyo, Japan), then fixed in 4% polyformaldehyde for 30 min, blocked with 3% FBS, incubated with primary antibodies against vascular endothelial cadherin (VE-cadherin) and vimentin overnight at 4 C, and then labeled with second antibody (donkey anti-mouse IgG, Alexa Fluor 555

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doi:10.5056/jnm18162

169 Moreover, knockdown of PKC- in rats leads to protection from fat-induced hepatic IR, 170 these results confirm the distinct roles of PKC- and PKC- in fat-induced IR in skeletal muscles and livers, respectively