After isotope accumulation, the choroid plexuses were rinsed in ice-cold isotope-free HEPES-aCSF containing 2 mM ouabain, 20 M bumetanide (Sigma, B3023
After this article was originally published, Novo Nordisk voluntarily dismissed its patent lawsuit against Hims & Hers without prejudice
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The general principles, including bacteriostatic versus sterile water and the surface-adsorption losses that matter at low concentrations, are covered in the peptide reconstitution guide